Banai was born in Beersheba and raised in Omer. Her father was a judge in Beersheba, and her mother was an education executive for the City of Beersheba. Many of her relatives are successful Israeli actors and singers. The next generation of the Banai family, including Orna and her brothers, Meir and Eviatar, followed this tradition. After three years of studying acting in Nissan Nativ's studio in Tel Aviv, Banai started performing in stand-up comedy and entertainment shows.
Banai became known for her portraying "Limor" in the humorous Israeli television shows Action and Rak BeIsrael (with Erez Tal). Since 2003, Banai has appeared in the popular satirical television show Eretz Nehederet. In addition, Banai acted in television dramas like Merchav-Yarkon and Max VeMoris. Banai also appeared in theatrical plays like Singles and The Last Striptease.
- Shapira, Michal (2008-12-03). "אורנה בנאי פורשת מהפוליטיקה" [Orna Banai quits politics]. Maariv. Retrieved 2018-02-17 – via Makor Rishon.
.חברת מפלגת הירוקים החליטה לפרוש מהמפלגה ומהפוליטיקה ולא להשתתף בבחירות המקומיות שיתקיימו בנובמבר השנה [The member of the Green Party has decided to quit the party and politics and will not participate in the local elections that will take place in November this year.]
- Arad, Dafna (2014-07-22). "Israeli artists opposing the war come under attack on social networks". Haaretz. Retrieved 2014-07-22.